What is 10X Phosphate Buffered Saline (PBS)?

10X Phosphate Buffered Saline (PBS) is a concentrated buffer solution used to maintain the pH and osmotic balance in biological applications. This solution is crucial in various laboratory protocols, including cell culture, protein assays, and molecular biology experiments. The '10X' indicates that it should be diluted to 1X concentration for actual use. PBS helps to stabilize the cellular environment, ensuring optimal conditions during experiments and providing a reliable medium for enzyme activity, cell suspension, or various biochemical reactions.

What are the common applications of 10X PBS?

10X PBS is widely used in the fields of molecular biology and biochemistry. Its primary applications include serving as a buffer for enzyme reactions, diluting cells in culture, and preparing common reagents for various assays. Researchers also utilize PBS for washing cells in flow cytometry and microscopy procedures. Its balanced pH and ionic composition make it an ideal medium for maintaining cell viability and function during manipulation, making it a staple in laboratory settings.

How do I prepare 1X Phosphate Buffered Saline from 10X PBS?

To prepare 1X PBS from 10X PBS, simply dilute the concentrated solution by mixing 100 mL of 10X PBS with 900 mL of distilled water. This will achieve the necessary concentration for most biological applications. It is crucial to use sterile water if the PBS will be applied in cell culture or sensitive experimental assays, to prevent contamination. Proper dilution ensures that the buffer maintains both its chemical integrity and effectiveness as a preserving solution in your experiments.

What is the pH of 10X PBS, and why is it important?

10X PBS typically has a pH of 7.4, which is close to the physiological pH of human blood and tissues. This pH level is vital because many biological processes are sensitive to pH variations. Maintaining a stable pH within this range helps to preserve enzyme activity, facilitate accurate assay results, and maintain cellular functions during experimental procedures. pH stability is especially crucial when working with proteins and nucleic acids, as even minor fluctuations can lead to significant changes in their behavior and functionality.

Is 10X PBS suitable for use in in-vivo studies?

10X PBS is primarily intended for in-vitro use, but it can play a role in in-vivo studies when appropriately diluted to 1X formulation for preparing buffers or diluents that need to be injected into living organisms. However, before any in-vivo application, researchers should ensure that filtration and sterilization processes are followed to eliminate any potential contaminants. Careful consideration of its components and the physiological impact on the organism should be undertaken for any application requiring in-vivo use.

Can 10X PBS be used for cell freezing applications?

Yes, 10X PBS can be utilized in cell freezing protocols when combined with cryoprotectants like dimethyl sulfoxide (DMSO) or glycerol. When preparing cells for freezing, it’s essential to first dilute the 10X PBS to 1X concentration, then mix it with appropriate cryoprotectants. This mixture helps to maintain cell viability and prevent ice crystal formation during the freezing process, which can damage cells. Researchers often use this method to preserve cell lines for future experiments and analyses.

What should I consider when storing 10X PBS?

10X PBS should be stored in a cool, dry place away from direct sunlight to maintain its stability and effectiveness. It is recommended to store it at 2-8 degrees Celsius. Proper sealing of the container is also vital to prevent contamination and evaporation. Before using any stored PBS, inspect it for signs of contamination or cloudiness, as these can affect experimental integrity. Proper storage ensures that the buffer retains its chemical properties for optimal use in research.

Is 10X PBS compatible with all cell types?

While 10X PBS is generally compatible with a wide range of cell types, the specific requirements may depend on the characteristics of the cells you’re working with. For instance, certain cells may have unique osmotic needs or require specific nutrients that are not provided by PBS. It is advisable to review the specific requirements of your cell line to ensure optimal growth conditions. Conducting preliminary tests can help determine the best approach before large-scale experiments.

How can I buy 10X Phosphate Buffered Saline (PBS), 7.4 pH, 1 L?

If you are looking to purchase 10X Phosphate Buffered Saline (PBS), 7.4 pH, 1 L in Argentina, you can easily find it on Ubuy. Ubuy offers a reliable platform for acquiring laboratory reagents and equipment, ensuring that you have access to quality products for your research needs. Their extensive catalog provides a convenient shopping experience, allowing you to select the right items tailored to your laboratory requirements.

¿Cómo comprar 10X Phosphate Buffered Saline (PBS), 7.4 pH, 1 L en línea desde Ubuy?

Es fácil comprar 10X Phosphate Buffered Saline (PBS), 7.4 pH, 1 L en línea desde Ubuy.. Solo tiene que buscar el producto, elegir su método de envío al pagar y recibirlo en su ubicación.

¿Está 10X Phosphate Buffered Saline (PBS), 7.4 pH, 1 L disponible para comprar en línea en Argentina?

Sí, en Ubuy Argentina, este producto está disponible para que lo compre a un precio razonable.. El 10X Phosphate Buffered Saline (PBS), 7.4 pH, 1 L no está disponible localmente, pero puede confiar en nosotros con nuestros servicios de envío exprés.

¿Cuánto tiempo se tarda en obtener el producto después de realizar el pedido?

El tiempo de entrega de su producto pedido varía según lo que haya pedido y el método de envío que haya elegido.. El tiempo de entrega estimado se menciona durante el proceso de pago, así que no se preocupe mientras compra.

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Optimal pH Balance

Maintains a stable pH of 7.4, ensuring reliable results in biological experiments and cell culture applications.

High Concentration

10X concentration allows for versatile dilution options, making it economical and suitable for various laboratory protocols.

Ready to Use

Conveniently packaged in 1 L, eliminating preparation time and ensuring consistency across multiple experiments for reliable outcomes.

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Brand Name	eCon Lab Supply
Manufacturer	Ciro Mfg
Included Components	Bottle
Part Number	23.105.04
Item Weight	1.5 pounds (680 grams)
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Cell Culture
Ideal for maintaining and growing mammalian cells in vitro, providing a stable pH and osmotic balance.
Laboratory Research
Great for various biochemical assays and experiments that require a buffered saline for maintaining biological systems.
Dilution Solutions
Perfect for preparing dilutions of biological samples or reagents needing a stable environment for effective results.

Non-biological Use
Not suitable for applications outside of biological research or laboratory settings, limiting its versatility.

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Pregunta: ¿Cómo comprar 10X Phosphate Buffered Saline (PBS), 7.4 pH, 1 L en línea desde Ubuy?

Respuesta: Es fácil comprar 10X Phosphate Buffered Saline (PBS), 7.4 pH, 1 L en línea desde Ubuy.. Solo tiene que buscar el producto, elegir su método de envío al pagar y recibirlo en su ubicación.
Pregunta: ¿Está 10X Phosphate Buffered Saline (PBS), 7.4 pH, 1 L disponible para comprar en línea en Argentina?

Respuesta: Sí, en Ubuy Argentina, este producto está disponible para que lo compre a un precio razonable.. El 10X Phosphate Buffered Saline (PBS), 7.4 pH, 1 L no está disponible localmente, pero puede confiar en nosotros con nuestros servicios de envío exprés.
Pregunta: ¿Cuánto tiempo se tarda en obtener el producto después de realizar el pedido?

Respuesta: El tiempo de entrega de su producto pedido varía según lo que haya pedido y el método de envío que haya elegido.. El tiempo de entrega estimado se menciona durante el proceso de pago, así que no se preocupe mientras compra.

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características y beneficios

Comprehensive range of biological buffers for research, diagnostics, and drug development.
Manufactured in an ISO 9001 and 13485 certified facility in the USA.
Produced under stringent Good Manufacturing Practices.
10X Phosphate Buffered Saline (PBS) is filter-sterilized for high purity.
Isotonic salt solution ideal for various cell culture applications.
Offered in convenient 500 mL and 1L sizes.

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